Journal
MECHANISMS OF DEVELOPMENT
Volume 121, Issue 9, Pages 1137-1143Publisher
ELSEVIER
DOI: 10.1016/j.mod.2004.05.013
Keywords
chick embryos; in ovo; electroporation; siRNA; gain-of-function; loss-of-function
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It remained very difficult to manipulate gene expression in chick embryos until the advent of in ovo electroporation which enabled the induction of both gain-of-function, and recently loss-of-function, of a gene of interest at a specific developmental stage. Gain-of-function by electroporation is so effective that it has become widely adopted in developmental studies in the chick. Recently, it became possible to induce loss-of-function by introducing an siRNA expression vector by electroporation. In this review, the methods of electroporation for gain-of-function and for loss-of-function by siRNA are discussed. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
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