Journal
JOURNAL OF BACTERIOLOGY
Volume 186, Issue 17, Pages 5945-5949Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.186.17.5945-5949.2004
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Funding
- NIAID NIH HHS [AI07422, T32 AI007422, R01 AI042347, R37 AI042347, AI42347] Funding Source: Medline
- NIDDK NIH HHS [P30DK-34928, P30 DK034928] Funding Source: Medline
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Transfer of SXT, a Vibrio cholerae-derived integrating conjugative element that encodes multiple antibiotic resistance genes, is repressed by SetR, a lambda(434) cI-related repressor. Here we identify divergent promoters between s086 and setR that drive expression of the regulators of SXT transfer. One transcript encodes the activators of transfer, setC and setD. The second transcript codes for SetR and, like the cl transcript of lambda, is leaderless. SetR binds to four operators located between setR and s086; the locations and relative affinities of these sites suggest a model for regulation of SXT transfer.
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