Journal
CELL COMMUNICATION AND ADHESION
Volume 11, Issue 5-6, Pages 137-153Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15419060500242836
Keywords
transdifferentiation; signal transduction; extracellular matrix; stem cells
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Funding
- NIBIB NIH HHS [5R01EB002197-02] Funding Source: Medline
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Adhesion to the extracellular matrix (ECM) proteins collagen I and vitronectin is sufficient to drive human mesenchymal stem cells (hMSCs) into an osteogenic differentiation pathway, but the mechanisms underlying this stimulation are not well understood. We found that addition of beta(1) and alpha(v)beta(3) integrin blocking antibodies inhibited ECM-induced ERK activation, while addition of the MEK inhibitor PD98059 blocked ERK activation, serine phosphorylation of the osteogenic transcription factor runx2/cbfa-1, osteogenic gene expression, and calcium deposition. These results suggest that ERK plays an important role in driving the ECM-induced osteogenic differentiation of hMSC.
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