Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 27, Issue 5, Pages 517-526Publisher
ELSEVIER GMBH
DOI: 10.1078/0723202041748190
Keywords
Burkholderia pyrrocinia; Burkholderia cepacia complex; polyphasic taxonomy
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Twenty-five Burkholderia cepacia-like isolates of human and environmental origin, comprising five different recA RFLP types, were examined by using a polyphasic taxonomic approach, including recA gene sequence analysis, 16S rRNA gene sequence analysis, DNA:DNA hybridisation studies, tDNA-PCR, fatty acid analysis and biochemical analysis. The results of the present study demonstrated that twenty-three of these strains belong to Burkholderia pyrrocinia, a B. cepacia complex species thus far comprising one single soil isolate only. An emended description of Burkholderia pyrrocinia is proposed. The taxonomic status of the remaining two isolates requires further analysis.
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