Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 429, Issue 1, Pages 23-29Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2004.05.014
Keywords
DOCK180; ELMO; guanine nucleotide exchange factor; molecular complex; nuclear localization
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DOCK180 protein plays a key role during development, cell motility, and phagocytosis. It forms a complex with another protein ELMO, and this complex acts as a guanine nucleotide exchange factor (GEF) for Rac. However, DOCK180-containing complexes have not been purified by unbiased biochemical approaches, and the nature and subcellular localization of these complexes remain unclear. Here, we show that a large fraction of endogenous DOCK180 is present as a 700kDa nuclear complex with ELMO proteins. In addition, this nuclear DOCK180/ELMO complex has functional Rac-GEF activity. Furthermore, endogenous DOCK180 could be found in complexes with different ELMO isoforms (ELMO1, 2 or 3) in different cell lines, depending on the ELMO isoforms expressed. These studies suggest that DOCK180 may associate with different ELMO proteins to form cell-type specific complexes and may have functions in both the nucleus and the cytoplasm. (C) 2004 Elsevier Inc. All rights reserved.
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