Journal
MOLECULAR ECOLOGY NOTES
Volume 4, Issue 3, Pages 474-476Publisher
WILEY
DOI: 10.1111/j.1471-8286.2004.00675.x
Keywords
cross-amplification; directional mutation; green swordtail; guppy
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The green swordtail (Xiphophorus hellerii; Poeciliidae) is a popular ornamental freshwater fish species. Fourteen microsatellites were isolated from a green swordtail genomic library enriched for CA-repeats. Thirteen microsatellites were polymorphic in green swordtail; interestingly four of them were tetranucleotide-type. Cross-species amplification showed that 10 of the 14 microsatellites were polymorphic in guppy (Poecilia reticulata; Poeciliidae) as well. No significant directional difference of allele length was seen between the two species at any of the loci tested.
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