Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 342, Issue 1, Pages 31-41Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2004.07.031
Keywords
engineering; endonuclease; specificity; crystal structure
Categories
Funding
- NCI NIH HHS [CA88942] Funding Source: Medline
- NIGMS NIH HHS [R15 GM069485-01, GM49857] Funding Source: Medline
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Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity. (C) 2004 Elsevier Ltd. All rights reserved.
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