Cellulose derivative-based beads as chiral stationary phase for HPLC

Crosslinked beads, which can be used as a chiral packing material (CPM) for high-performance liquid chromatography (HPLC), were prepared using a cellulose phenylcarbamate derivative. The spherical beads were first prepared with partially derivatized cellulose 3,5-dimethylphenylcarbamate, which was then crosslinked with a diisocyanate. The obtained beads had a higher loading capacity than the corresponding conventional CPM prepared by coating the derivative on silica gel, and were not damaged when used with an eluent containing chloroform which cannot be used with the coated CPM.