Journal
OPTICS EXPRESS
Volume 12, Issue 18, Pages 4203-4213Publisher
OPTICAL SOC AMER
DOI: 10.1364/OPEX.12.004203
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Subcellular organelles in living cells were inactivated by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of a mitochondrion in living cells was experimentally confirmed by stacking three-dimensional confocal images and by restaining of organelles. The viability of the cells after femtosecond laser irradiation was ascertained by impermeability of propidium iodide as well as by the presence of cytoplasmic streaming. (C) 2004 Optical Society of America.
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