Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 70A, Issue 4, Pages 603-614Publisher
WILEY
DOI: 10.1002/jbm.a.30122
Keywords
biodegradable; elastomer; scaffold; poly(ester urethane) urea; electrospinning; collagen
Funding
- NHLBI NIH HHS [R01 HL069368, R01 HL069368-02, R01 HL069368-01A1, HL069368] Funding Source: Medline
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The native extracellular matrix (ECM) of elastic tissues is strong and flexible and supports cell adhesion and enzymatic matrix remodeling. In an attempt to convey these ECM properties to a synthetic scaffold appropriate for soft tissue engineering applications, a biodegradable, elastomeric poly(ester urethane)urea (PEUU) was combined with type I collagen at various ratios (2.5, 5, 10, 20, 50, 60, 70, 80, and 90 wt% collagen) and electrospun to construct elastic matrices. Randomly orientated fibers in the electrospun matrices ranged in diameter from 100-900 nm, dependent on initial polymer concentration. Picrosirius red staining of matrices and CD spectroscopy of released collagen confirmed collagen incorporation and preservation of collagen structure at the higher collagen mass fractions. Matrices were strong and distensible possessing strengths of 2-13 MPa with breaking strains of 160-280% even with low PEUU content. Collagen incorporation significantly enhanced smooth muscle cell adhesion onto electrospun scaffolds. An approach has been demonstrated that mimics elastic extracellular matrices by using a synthetic component to provide mechanical function together with a biomacromolecule, collagen. Such matrices may find application in engineering soft tissue. (C) 2004 Wiley Periodicals, Inc.
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