Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 200, Issue 6, Pages 811-816Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20040776
Keywords
HLH; cytotoxic granule; NK cell; CTL; immunodeficiency
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About 30% of cases of the autosomal recessive immunodeficiency disorder hemophagocytic lymphohistiocytosis are believed to be caused by inactivating mutations of the perform gene. We expressed perform in rat basophil leukemia cells to define the basis of perform dysfunction associated with two mutations, R225W and G429E, inherited by a compound heterozygote patient. Whereas RBL cells expressing wild-type perform (67 kD) efficiently killed Jurkat target cells to which they were conjugated, the substitution to tryptophan at position 225 resulted in expression of a truncated (similar to45 kD) form of the protein, complete loss of cytotoxicity, and failure to traffic to rat basophil leukemia secretory granules. By contrast, G429E perform was correctly processed, stored, and released, but the rat basophil leukemia cells possessed reduced cytotoxicity. The defective function of G429E perform mapped downstream of exocytosis and was due to its reduced ability to bind lipid membranes in a calcium-dependent manner. This study elucidates the cellular basis for perform dysfunctions in hemophagocytic lymphohistiocytosis and provides the means for studying structure-function relationships for lymphocyte perform.
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