Determination of dopamine by flow-injection analysis coupled with luminol-hexacyanoferrate(III) chemiluminescence detection

A novel flow-injection method (FIA) for the determination of dopamine based on the inhibition of the intensity of chemiluminescence (CL) from luminol-hexacyanoferrate(III) system in basic medium is described. The present method allows the determination of dopamine over the range 30-100 mug l(-1) and 400-3000 mug l(-1). The relative standard deviation is 2.32% for 70 mug l(-1) dopamine and 1.22% for 1500 mug l(-1) dopamine (n = 20). The detection limit is 5 mug l(-1) with the sampling rate of 135 samples h(-1). This method has been applied for the determination of dopamine in commercial pharmaceutical injection samples. The results obtained by this method agreed with those by the official method. (C) 2004 Elsevier B.V. All rights reserved.