Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 101, Issue 39, Pages 14036-14039Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0406115101
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- NIA NIH HHS [AG18231] Funding Source: Medline
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We find that single- and double-stranded oligonucleotides have different propensities to adsorb on gold nanoparticles in colloidal solution. We use this observation to design a hybridization assay based on color changes associated with gold aggregation. Because the underlying adsorption mechanism is electrostatic, no covalent functionalization of the gold, the probe, or the target DNA is required. Hybridization conditions can be optimized because it is completely separated from the detection step. The assay is complete within 5 min, and < 100 femtomoles of target produces color changes observable without instrumentation. Single-base-pair mismatches are easily detected.
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