Journal
ELECTROPHORESIS
Volume 25, Issue 18-19, Pages 3128-3131Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200406020
Keywords
biomarkers; cross-injector; glycomics; lab-on-a-chip; microfluidics; miniaturization
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We implemented 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled asparagine-linked glycan (N-glycan) profiling on a microfluidic electrophoresis platform. Using 11.5 cm effective length etched channels and 4% linear polyacrylamide as the separation matrix, the major N-glycans in human serum were profiled in 12 min with a resolution comparable to what is achieved for these analytes on gel-based DNA sequencers. This demonstration suggests a practical clinical application for highspeed compact analyzers which might be uniquely based on microfluidic devices.
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