Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 59, Issue 1, Pages 109-115Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2004.06.010
Keywords
cell density; fluorescent in situ hybridisation; Lactobacillus plantarum; single cell activity
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Fluorescent in situ hybridisation (FISH) with a 16S ribosomal RNA (rRNA)-targeted oligonucleotide probe, Eub338, could be used to estimate the in situ activity of Lactobacillus plantarum WCFS1 in exponentially growing cells. However, L. plantarum is capable of growth to very high cell densities, and the properties of the L. plantarum cell envelope prevented effective entry of the fluorescent oligonucleotide probe into the cells at later stages of growth at high cell densities. Total rRNA measurements of cells isolated at different growth stages showed maximal amounts of RNA (8.77 +/- 0.8 fg) per cell at the early stationary phase and confirmed the effectiveness of FISH for accurate activity measurement in exponentially growing cells. (C) 2004 Elsevier B.V. All rights reserved.
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