Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 121, Issue 1, Pages 85-91Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2004.06.006
Keywords
SARS; coronavirus; virus inactivation; tissue culture
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Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat treatment of 65 degreesC or greater, alkaline (pH > 12) or acidic (pH < 3) conditions, formalin and glutaraldehyde treatments. We describe the kinetics of these efficient viral inactivation methods, which will allow research with SARS-CoV containing materials, that are rendered non-infectious, to be conducted at reduced safety levels. (C) 2004 Elsevier B.V. All rights reserved.
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