Journal
MICROCHEMICAL JOURNAL
Volume 78, Issue 2, Pages 135-142Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.microc.2004.03.018
Keywords
capillary electrophoresis; NDA derivatization; laser-induced fluorescence; saliva samples; substance P
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An analytical method was developed to determine the undecapetide substance P (SP) based on capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. SP was derivatized with the fluorogenic reagent 2,3-naphthalenedicarboxaldehyde (NDA) prior to injection into the CE-LIF system. The pre-column derivatization scheme combined with injection enhancement techniques extends the delectability of SP to the subnanomolar level. Limit of detection (LOD) of 100 pM was achieved without pre-concentrating the sample prior to injection. The reproducibility for six different preparations of a standard sample containing 5 nM of SP was 6.8% RSD and that of the CE migration time was 0.08% RSD. The method was used to determine SP in a saliva sample. (C) 2004 Elsevier B.V. All rights reserved.
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