Journal
ANALYTICAL CHEMISTRY
Volume 76, Issue 19, Pages 5960-5964Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac049300f
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We report here the development of a device for single-molecule imaging on large surface areas. A CCD camera operated in time delay and integration mode is synchronized with the movement of a sample scanning stage, enabling continuous data acquisition. Experiments on single fluorescent lipid molecules in supported lipid bilayers and on stained living cells demonstrate the capabilities of the method. Areas of up to 5 x 5 mm(2) were recorded within 11 min at a pixel size of 129 nm.
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