Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 16, Issue 5, Pages 395-400Publisher
SPRINGER
DOI: 10.1023/B:JAPH.0000047950.76549.ce
Keywords
Dunaliella salina; nitrate transporter gene; nitrate; salt stress
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A nitrate transporter gene, named DsNRT2.1 (GeneBank accession number AY621079), from Dunaliella salina has been cloned by screening a cDNA library, which was constructed with mRNAs from D. salina after 60 min treatment with 5 mM nitrate, with a 342 bp NRT2 cDNA fragment from D. salina as a probe. DsNRT2.1 exhibits sequence similarity to those known nitrate transporters of the NRT2 family. A hydrophobicity blot indicated that DsNRT2.1 belongs to the major facilitator superfamily (MFS). Northern analysis showed that an mRNA species of 1.9 kb can be rapidly induced by NO3-, but not by NH4+. Northern analysis also showed that NaCl could significantly increase the expression of DsNRT2.1.
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