Journal
APPLIED PHYSICS LETTERS
Volume 103, Issue 3, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4813822
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Funding
- Theme-based Research Scheme [T13-706]
- Research Grants Council of the Hong Kong Special Administrative Region, China [CityU 120310]
- University Grants Council (UGC) Special Equipment Grant [SET_CityU 01]
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We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and fused under irradiation with pulsed UV laser at 355 nm. Successful fusion was indicated by green fluorescence protein transfer. The influence of laser pulse energy on the fusion efficiency was investigated. The fused products were viable as gauged by live cell staining. Successful fusion of hESCs with somatic cells was also demonstrated. The reported fusion outcome may facilitate studies of cell differentiation, maturation, and reprogramming. (C) 2013 AIP Publishing LLC.
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