Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1050, Issue 2, Pages 211-216Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2004.08.049
Keywords
oligosaccharide; capillary electrophoresis; 3-aminobenzoic acid; 8-aminopyrene-1,3,6-trisulfonate; antibody pharmaceutical
Ask authors/readers for more resources
Carbohydrate chains in glycoprotein pharmaceuticals have important roles for the expression of their biological activities. Therefore, development of an assessment method for the carbohydrate chains is an important parameter for quality control of glycoprotein pharmaceuticals such as newly developed therapeutic antibodies. In this report, we applied capillary electrophoresis with laser-induced fluorescence detection to the analysis of carbohydrate chains after releasing with glycoamidase followed by derivatization with 3-aminobenzoic acid. We found that four major oligosaccharides present in antibody pharmaceuticals were successfully separated with good resolution. The present method showed good precision in both migration times and relative peak areas, and gave comparable accuracy with that using a derivatization method with 8-aminopyrene-1,3,6-trisulfonate. (C) 2004 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available