Journal
JOURNAL OF ETHNOPHARMACOLOGY
Volume 94, Issue 2-3, Pages 279-281Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.jep.2004.06.013
Keywords
antibacterial; bioassay; medicinal plant; Peperomia galioides
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Funding
- NIGMS NIH HHS [1 R15 GM59049-01] Funding Source: Medline
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A versatile microplate bioassay for quick and sensitive determination of antibacterial activity was developed for use in screening medicinal plants and identification of their active principles. This assay can be used to determine minimum inhibitory concentrations for small quantities of organic or water-soluble plant extracts. Bioassay-guided fractionation of the stem and leaves of Peperomia galioidcs using this method found fractions containing grifolin and grifolic acid, which inhibited growth of Staphylococcus aureus and Staphylococcus epidermidis. (C) 2004 Published by Elsevier Ireland Ltd.
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