Journal
RESEARCH IN MICROBIOLOGY
Volume 155, Issue 8, Pages 605-610Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2004.05.002
Keywords
Bacillus subtilis; transcription; protein folding; translocation; proteolysis
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Bacillus subtilis is an alternative host for expression and secretion of heterologous proteins. However, low yields of protein production limit its use on a wide scale. The secretory pathway of proteins can be divided into three functional stages: the early stage, involving the synthesis of secretory pre-proteins, their interaction with chaperones and binding to the secretory translocase; the second stage, translocation across the cytoplasmic membrane; and the last stage, including removal of the signal peptide, protein refolding and passage through the cell wall. Five bottlenecks for expression and secretion of heterologous proteins are described in this review: transcription, protein folding, translocation, signal peptide processing and proteolysis. (C) 2004 Elsevier SAS. All rights reserved.
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