Journal
NATURE IMMUNOLOGY
Volume 5, Issue 10, Pages 1078-1087Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ni1121
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Funding
- NCI NIH HHS [CA81776, CA96547] Funding Source: Medline
- NIAID NIH HHS [AI56363] Funding Source: Medline
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The interaction of CD22 with alpha2,6-linked sialic acid ligands has been widely proposed to regulate B lymphocyte function and migration. Here, we generated gene-targeted mice that express mutant CD22 molecules that do not interact with these ligands. CD22 ligand binding regulated the expression of cell surface CD22, immunoglobulin M and major histocompatibility complex class II on mature B cells, maintenance of the marginal zone B cell population, optimal B cell antigen receptor-induced proliferation, and B cell turnover rates. However, CD22 negative regulation of calcium mobilization after B cell antigen receptor ligation, CD22 phosphorylation, recruitment of SHP-1 to CD22 and B cell migration did not require CD22 ligand engagement. These observations resolve longstanding questions regarding the physiological importance of CD22 ligand binding in the regulation of B cell function in vivo.
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