Journal
PLANT JOURNAL
Volume 40, Issue 1, Pages 164-172Publisher
WILEY
DOI: 10.1111/j.1365-313X.2004.02195.x
Keywords
in vitro transcription; plastid-encoded RNA polymerase (PEP); PEP-associated protein; plastid sigma factor; tobacco
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We affinity-purified the tobacco plastid-encoded plastid RNA polymerase (PEP) complex by the alpha subunit containing a C-terminal 12 x histidine tag using heparin and Ni2+ chromatography. The composition of the complex was determined by mass spectrometry after separating the proteins of the >900 kDa complex in blue native and SDS polyacrylamide gels. The purified PEP contained the core alpha, beta, beta', beta subunits and five major associated proteins of unknown function, but lacked sigma factors required for promoter recognition. The holoenzyme efficiently recognized a plastid psbA promoter when it was reconstituted from the purified PEP and recombinant plastid sigma factors. Reconstitution of a plastid holoenzyme with individual sigma factors will facilitate identification of sigma factor-specific promoter elements.
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