Journal
BIOTECHNIQUES
Volume 37, Issue 4, Pages 598-+Publisher
FUTURE SCI LTD
DOI: 10.2144/04374ST04
Keywords
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Funding
- NCI NIH HHS [CA62924] Funding Source: Medline
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Current DNA electrophoretic solutions employ high ionic concentrations and require long electrophoretic run times. Here we demonstrate that high and low molecular weight double-stranded DNA, single-stranded DNA (ssDNA), and RNA can be separated rapidly in agarose-based low-molarity conductive media. Separation of small DNA fragments was optimized by substituting 1-mM solutions of alkali metals or a nonbiological arnine that distributed voltage with a minute current. These ultra-dilute solutions can separate DNA at least 15-fold faster. Low-molarity media at 5-10 mM adequately separated RNA and larger DNA fragments as well. These novel media reduce the Joule heating of the electrophoretic system and allow for easy-to-use, ultra-fast separation of DNA fragments.
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