Effective inhibition of HIV-1 replication in cultured cells by external guide sequences and ribonuclease P

We examined the suppressive effect of HIV-1 RNA gene cleavge on HIV-1 expression, using the catalytic RNA subunit RNase P and the 3'-half tRNA(Try) [external guide sequence (EGS)] in cultured cells. HIV-1 expression was inhibited by the tRNA(met)-EGS-U5 and U6-EGS-U5 from the tRNA(met) and U6 promoters, respectively. There was no difference in the inhibitory effects on HIV-1 expression between the tRNA(met) and U6 promoters. (C) 2004 Elsevier Ltd. All rights reserved.