Journal
NEUROREPORT
Volume 15, Issue 14, Pages 2181-2184Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200410050-00007
Keywords
caffeic acid; 5-lipoxygenase; minocycline; oxygen-glucose deprivation (OGD); rat pheochromocytoma (PC12) cells
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Minocycline protects animals against cerebral ischemia by inhibiting inflammation. To determine whether minocycline protects PC12 cells from in vitro ischemic-like injury and affects pro-inflammatory 5-lipoxygenase activation, the cell viability and 5-lipoxygenase translocation to nuclear membrane were observed after oxygen-glucose deprivation (OGD). We found that OGD reduced cell viability, which was attenuated by minocycline and 5-lipoxygenase inhibitor caffeic acid. 5 -Lipoxygenase protein was detected in PC12 cells by immunohistochemical and Western blot analyses. OGD induced 5-lipoxygenase translocation to nuclear membranes, which was abolished by minocycline and caffeic acid. Thus, minocycline can protect PC12 cells from in vitro ischemic-like injury, and this effect may be partly related to the inhibition of 5-lipoxygenase activation.
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