4.7 Article

A role for talin in presynaptic function

Journal

JOURNAL OF CELL BIOLOGY
Volume 167, Issue 1, Pages 43-50

Publisher

ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200406020

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Funding

  1. NCI NIH HHS [P01 CA046128, CA 46128] Funding Source: Medline
  2. NIMH NIH HHS [F32 MH 067385, F32 MH067385] Funding Source: Medline
  3. NINDS NIH HHS [R37 NS036251, NS 36251, R01 NS036251, NS 037823] Funding Source: Medline

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Talin, an adaptor between integrin and the actin cytoskeleton at sites of cell adhesion, was recently found to be present at neuronal synapses, where its function remains unknown. Talin interacts with pkosphatidylinositol-(4)-phosphate 5-kinase type Igamma, the major phosphatidylinositol-(4,5)-bisphosphate [Pl(4,5)P-2]-synthesizing enzyme in brain. To gain insight into the synaptic role of talin, we microinjected into the large lamprey axons reagents that compete the talin-PIP kinase interaction and then examined their effects on synaptic structure. A dramatic decrease of synaptic actin and an impairment of clathrin-mediated synaptic vesicle endocytosis were observed. The endocytic defect included an accumulation of clathrin-coated pits with wide necks, as previously observed after perturbing actin at these synapses. Thus, the interaction of PIP kinase with talin in presynaptic compartments provides a mechanism to coordinate Pl(4,5)P-2 synthesis, actin dynamics, and endocytosis, and further supports a functional link between actin and clathrin-mediated endocytosis.

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