Journal
FEMS MICROBIOLOGY LETTERS
Volume 239, Issue 2, Pages 277-283Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2004.08.045
Keywords
Sinorhizobium meliloti; Bradyrhizobium japonicum; polyhydroxyalkanoate synthesis; heterologous complementation; exopolysaccharide
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A reduced exopolysaccharide phenotype is associated with inability to synthesize polyhydroxyalkanaote (PHA) stores in Sinorhizobium meliloti strain Rm1021. Loss of function mutations in phbB and phbC result in non-mucoid colony morphology on Yeast Mannitol Agar, compared to the mucoid phenotype exhibited by the parental strain. This phenotype is attributed to reduction in succinoglycan synthesis. We have used complementation of this phenotype and the previously described D-3-hydroxybutyrate/acetoacetate utilization phenotype to isolate a heterologous clone containing a Bradyrhizobium japonicum phbC gene. Sequence analysis confirmed that this clone contains one of the five predicted phbC genes in the B. japonicum genome. The described phenotypic complementation strategy should be useful for isolation of novel PHA synthesis genes of diverse origin. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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