Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1052, Issue 1-2, Pages 223-227Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2004.08.127
Keywords
high-speed counter-current chromatography; Angelica dahurica (Fisch ex Hoffm) Benth; et Hook. f; imperatorin; isoimperatorin; oxypeucedanine
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A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of coumarins from Angelica dahurica (Fisch. ex Hoffin) Benth, et Hook. f (Baizhi in Chinese) was successfully established by using n-hexane-methanol-water as the two-phase solvent system in gradient elution mode. The upper phase of n-hexane-methanol-water (5:5:5, v/v) was used as the stationary phase of HSCCC. The mobile phase used in HSCCC was the lower phase of n-hexane-methanol-water (5:5:5, v/v) and n-hexane-methanol-water (5:73, v/v) that was changed in gradient. Three major components including imperatorin, isoimperatorin and oxypeucedanine were isolated, each at over 98% purity as determined by high-performance liquid chromatography (HPLC). The peak fractions of HSCCC were identified by H-1-NMR and C-13-NMR. (C) 2004 Elsevier B.V. All rights reserved.
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