Journal
ANALYTICAL CHEMISTRY
Volume 76, Issue 20, Pages 6058-6063Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0493626
Keywords
-
Categories
Funding
- NIDDK NIH HHS [DK54932] Funding Source: Medline
Ask authors/readers for more resources
Experiments were performed to characterize the in vitro collection of macromolecules using microdialysis. Fluorescently labeled proteins and dextrans ranging from 3000 to 150 000 were sampled using a 10-mm, 100 000 molecular weight cutoff, polyethersulfone microdialysis probe. Published models describing microdialysis mass transport of small molecules were examined to determine their appropriateness for sampling of macromolecules. Collection efficiencies, reported as relative recoveries, for macromolecules from 3000 to 70 000 ranged from 5 to 44%. Collection efficiencies determined for microdialysis sampling of macromolecules follow the functionality of published models, although experimental mass transport resistances are to some extent smaller than predicted. Implications of the current study for in vivo microdialysis sampling of cytokines and growth factors are discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available