4.7 Article

Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

Journal

CHEMICAL COMMUNICATIONS
Volume -, Issue 20, Pages 2260-2261

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b411973h

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PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal Polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 1, heterologous expression of which led to the biosynthesis of the squalestatin side-chain.

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