Journal
FEBS LETTERS
Volume 576, Issue 3, Pages 423-427Publisher
WILEY
DOI: 10.1016/j.febslet.2004.09.028
Keywords
cDNA array; cadmium responding gene; ectomycorrhizal fungous; Paxillus involvutus
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The molecular mechanisms underlying the response of ectomycorrhizal fungi to heavy metals in general and cadmium in particular remain poorly understood. We screened 2040 arrayed cDNAs of the ectomycorrhizal fungus Paxillus involutus to identify cadmium-responsive genes by using differential hybridization. Forty nine (2.4%) of the 2040 cDNAs were differentially expressed, among which transcripts coding a laccase, an aconitase, and a metallothionein were upregulated by 3.9-, 3.7- and 2.8-fold, respectively, whereas genes coding hydrophobins and threonine dehydratase were strongly downregulated. Our results suggest that complexation of cadmium by phenolic compounds, or by complexing peptides such as metallothioneins, is probably key determinant of the cellular response to cadmium in P. involutus. In addition, the present study suggests that the synthesis of hydrophobins may be efficiently, reduced, thus redirecting Cys to the manufacture of Cys-enriched compounds. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V.. All rights reserved.
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