Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1053, Issue 1-2, Pages 101-106Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2004.06.125
Keywords
monolithic columns; stationary phases; LC; carbohydrates; latex
Funding
- NIGMS NIH HHS [GM-48364] Funding Source: Medline
Ask authors/readers for more resources
A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch. (C) 2004 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available