Calcium inhibits muscle FBPase and affects its intracellular localization in cardiomyocytes

As our recent investigation revealed, in mammalian heart muscle, fructose 1,6-bisphosphatase (FBPase) - a key enzyme of glyconeogenesis - is located around the Z-Iine, inside cell's nuclei and, as we demonstrate here for the first time, it associates with intercalated discs. Since the degree of association of numerous enzymes with subcellular structures depends on the metabolic state of the cell, we studied the effect of elevated Ca2+ concentration on localization of FBPase in cardiomyocytes. In such conditions, FBPase dissociated from the Z-Iine, but no visible effect on FBPase associated with intercalated discs or on the nuclear localization of the enzyme was observed. Additionally, Ca2+ appeared to be a strong inhibitor of muscle FBPase. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.