Journal
PROCESS BIOCHEMISTRY
Volume 39, Issue 12, Pages 2109-2114Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2003.11.001
Keywords
lipase; enzyme production; surfactants; fatty acids; triglycerides; carbon source
Ask authors/readers for more resources
The ability of the yeast Issatchenkia orientalis CECT 10688 to secrete lipolytic activity in submerged culture was investigated. The yeast was grown in a complex medium supplemented with a fixed concentration of several lipidic compounds (triglycerides, fatty acids). Maximum enzyme activity around 70-80 U cm(-3) was produced in cultures supplemented with tributyrin. An optimum tributyrin concentration of 10gdm(-3) was selected. Several surfactants were added to the cultures, but no significant increase in activity was detected. Finally, the effect of the type of carbon source on lipolytic enzyme production was studied. The best results were obtained with glucose and fructose (60-80 U cm(-3)), while rather low enzyme activity was found in cultures grown on lactose and maltose (about 20 U cm(-3)). (C) 2003 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available