Journal
JOURNAL OF PROTEOME RESEARCH
Volume 3, Issue 6, Pages 1210-1218Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr049886m
Keywords
2-D PAGE; missing data; normalization; mitochondria; permutation test; false discovery rates
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Funding
- NCI NIH HHS [CA P30 54174] Funding Source: Medline
- NEI NIH HHS [R01 EY 11733] Funding Source: Medline
- NIA NIH HHS [R01 AG 23843, P01 AG 20591, 1P30 AG 13319] Funding Source: Medline
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Several high-throughput statistical methods were evaluated for processing data generated by two-dimensional polyacrylamide gel electrophoresis, including how to handle missing data, normalization, and statistical analysis of data obtained from 2-D gels. Quantile normalization combined with a nonparametric permutation test based on minimizing false discover rates gave the highest yield of proteins that changed with genotype and detected the anticipated 50% decrease in Mn-superoxide dismutase (MnSOD) protein levels in mitochondrial extracts obtained from MnSOD-deficient mice.
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