Journal
ELECTROPHORESIS
Volume 25, Issue 21-22, Pages 3832-3839Publisher
WILEY
DOI: 10.1002/elps.200406091
Keywords
amperometric detection; microchip; miniaturization
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We describe the development of an electrophoresis microchip fabricated by a direct-printing process, based on lamination of printed polyester films with end-channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester-toner devices presented an electroosmotic flow (EOF) magnitude of similar to10(-5) cm(2)V(-1)s(-1), which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser-printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol(.)L(-1) (135 amol) and 1.8 mumol(.)L(-1) (486 amol) for iodide and ascorbate, respectively.
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