Journal
REPRODUCTIVE TOXICOLOGY
Volume 19, Issue 1, Pages 71-77Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2004.06.002
Keywords
4-vinylcyclohexene diepoxide; ovarian toxicant; ovarian follicle; in vitro; ovarian culture; ovotoxicity; delayed toxicity; single dose
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Funding
- NIEHS NIH HHS [ES06694, ES08979, ES09269] Funding Source: Medline
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Repeated daily dosing of rats with the ovotoxic, occupational chemical 4-vinylcyclohexene diepoxide (VCD, 80 mg/kg, i.p.) selectively depletes primordial and primary ovarian follicles. This study was designed to investigate whether follicle loss can be achieved following a single, acute exposure to VCD. Ovaries removed from postnatal-day-4 female Fischer 344 rats were cultured in the absence or presence of VCD for 15 days. Continuous in vitro exposure to VCD (15 days) caused concentration-dependent loss of primordial and small primary follicles. A single exposure to VCD in vitro (30 muM, 24 h) also caused significant losses of primordial and primary follicles 14 days later. Additionally, 28-day-old female rats were given a single injection of VCD (40-320 mg/kg, i.p.). A single dose at 320 mg/kg resulted in substantial loss of all follicle stages beginning 6 days later. Overall, these results demonstrate that an acute exposure to high concentrations/doses of VCD is sufficient to cause subsequent delayed loss of follicles. (C) 2004 Elsevier Inc. All rights reserved.
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