Journal
EUROPEAN PHYSICAL JOURNAL E
Volume 15, Issue 3, Pages 277-286Publisher
SPRINGER
DOI: 10.1140/epje/i2004-10073-5
Keywords
-
Ask authors/readers for more resources
We describe a microfluidic all-optical technique to measure the thermophoresis of molecules. Within micrometer-thick chambers, we heat, aqueous solutions with a micrometer-sized focus of infrared light. The temperature increase of about 1 K. is monitored with temperature-sensitive fluorescent, dyes. We test the approach in measuring the thermophoresis of DNA. We image die concentration of DNA in a second fluorescence-color channel. DNA is depleted away from the heated spot. The profile of depletion is fitted by the thermophoretic theory to reveal the Soret coefficient. We evaluate the method with numerical 3D calculations of temperature profiles: drift,, convection and thermophoretic depletion using finite element methods. The approach opens new ways to monitor thermophoresis at the single molecule level. near boundaries and in complex mixtures. The flexible microfluidic setting is a good Step towards microfluidic applications of thermophoresis in biotechnology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available