Journal
BIOSENSORS & BIOELECTRONICS
Volume 20, Issue 4, Pages 668-683Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.06.032
Keywords
DNA microarrays; parallel detection tools; biodefense
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Funding
- NCRR NIH HHS [R01 RR018625] Funding Source: Medline
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Development of parallel detection tools using microarrays is critically reviewed in view of the need for screening multiple microorganisms in a single test. Potential research needs with respect to probe design and specificity, validation, sample concentration, selective target enrichment and amplification, and data analysis are discussed. Data illustrating selected probe design issues for detecting multiple targets in mixed microbial systems is presented. Challenges with respect to cost, time, and ease of use compared to other methods are also summarized. (C) 2004 Elsevier B.V. All rights reserved.
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