Journal
BIOTECHNOLOGY ADVANCES
Volume 22, Issue 8, Pages 633-658Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2004.06.003
Keywords
biofilm; bioprocess; cell physiology; gel entrapment; protein expression; proteomics
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Biotechnological processes based on immobilized viable cells have developed rapidly over the last 30 years. For a long time, basic studies of the physiological behaviour of immobilized cells (IC) have remained in the shadow of the applications. Natural IC structures, i.e. biofilms, are being increasingly investigated at the cellular level owing to their definite importance for human health and in various areas of industrial and environmental relevance. This review illustrates this paradoxical development of research on ICs, starting from the initial rationale for IC emergence and main application fields of the technology-with particular emphasis on those that exploit the extraordinary resistance of ICs to antimicrobial compounds-to recent advances in the proteomic approach of IC physiology. (C) 2004 Elsevier Inc. All rights reserved.
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