4.6 Article

Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 70, Issue 11, Pages 6884-6886

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.70.11.6884-6886.2004

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Funding

  1. Austrian Science Fund FWF [P 16260] Funding Source: Medline

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Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites.

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