Journal
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
Volume 57, Issue 3, Pages 133-141Publisher
WILEY
DOI: 10.1002/arch.20017
Keywords
Rhodnius prolixus; water channel; RP-MIP; insect; cAMP
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The purpose of the present study was to detect the presence of an aquaporin-like water channel, a member of the major intrinsic protein (MIP) family, in the Malpighian tubule (MT) of the hematophagous insect Rhodnius prolixus, which excrete a large bulk of fluid after a massive blood meal, and its possible regulation by 5-hydroxytryptamine (5-HT). Reverse transcription polymerose chain reaction (RT-PCR) and Southern blots of cDNA was obtained from adult R. prolixus MT poly (A)(+) RNA. Employing degenerate primers corresponding to the NPA (amino acid sequence motifs repeats Asn-Pro-Ala) highly conserved domain of amino acids sequences of all members of the MIP gene family, we were able to identify a 365-base pair PCR product. The R. prolixus MT mRNA expression of this water transporter is increased in the animal after blood meal and in tubules treated with 5-hydroxytryptamine or cAMP. The up-regulated expression of MT MIP mRNA after a blood meal is probably due to the action of 5-hydroxytryptamine via a cyclic AMP dependent pathway. Arch. Insect Biochem. Physiol. 57:133-141, 2004. (C) 2004 Wiley-Liss, Inc.
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