An autocrine mechanism for constitutive Wnt pathway activation in human cancer cells

Autocrine Writ signaling in the mouse mammary tumor virus model was the first identified mechanism of canonical pathway activation in cancer. In search of this transformation mechanism in human cancer cells, we identified breast and ovarian tumor lines with upregulation of the uncomplexed transcriptionally active form of beta-catenin without mutations afflicting downstream components. Extracellular Writ antagonists FRP1 and DKK1 caused a dramatic downregulation of P-catenin levels in these tumor cells associated with alteration of biological properties and increased expression of epithelial differentiation markers. Colorectal carcinoma cells with knockout of the mutant P-catenin allele retained upregulated P-catenin levels, which also could be inhibited by these Writ antagonists. Together, these findings establish the involvement of autocrine Writ signaling in human cancer cells.