Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 9, Issue 6, Pages 482-489Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/BF02933490
Keywords
Bacillus; wastewater treatment; fluorescence in situ hybridization (FISH); quantitative PCR
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Bacillus species were observed and quantified by molecular approaches, using the 16S rDNA primers/probes, in a wastewater treatment plant designed for the purpose of stimulating the growth of Bacillus species. The plant has been operating as a test plant since 1997 in the city of Ina, Japan, with excellent treatment performance. Observations by in situ hybridization, using Bacillus-specific probes, indicated that Bacillus strains were inhabited in the plant and their numbers decreased during the treatment process. Similar results were obtained from a quantitative PCR analysis using a Bacillus-specific primer set, and the amount of DNA originating from various Bacillus species was maximally 1.91% of the total DNA in the wastewater treatment tank. Clone library analysis using the Bacillus-specific primers suggested that, while the population was noticeably increased, the phylogenetic diversity of the increasing Bacillus species was very low.
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