Journal
JOURNAL OF IMMUNOLOGY
Volume 173, Issue 9, Pages 5671-5678Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.173.9.5671
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Funding
- NIAID NIH HHS [AI 48917, R01 AI048917-04A1] Funding Source: Medline
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Two fully human mAbs specific for epitopes dependent on intact carboxylate groups on the C6 carbon of the mannuronic acid components of Pseudomonas aeruginosa alginate were found to promote phagocytic killing of both mucoid and nomnucoid strains as well as protection against both types of strains in a mouse model of acute pneumonia. The specificity of the mAbs for alginate was determined by ELISA and killing assays. Some strains of P. aeruginosa did not make detectable alginate in vitro, but in vivo protection against lethal pneumonia was obtained and shown to be due to rapid induction of expression of alginate in the murine lung. No protection against strains genetically unable to make alginate was achieved. These mAbs have potential to be passive therapeutic reagents for all strains of P. aeruginosa and the results document that alginate is a target for the proper type of protective Ab even when expressed at low levels on phenotypically nonmucoid strains.
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