Journal
ORAL ONCOLOGY
Volume 40, Issue 10, Pages 1040-1047Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.oraloncology.2004.05.010
Keywords
non receptor tyrosine kinase; oral squamous cell carcinoma; BRK; Sik; PTK6
Categories
Funding
- NIDCR NIH HHS [DE00470, DE12322] Funding Source: Medline
- NIDDK NIH HHS [R01 DK044525, R01 DK044525-12] Funding Source: Medline
Ask authors/readers for more resources
BRK is a non-receptor tyrosine kinase whose functional role is poorly understood. Although it is an epithelial specific kinase, its expression appears to be tissue specific. To date, little is known about BRK expression in human oral epithelium. We investigated expression of BRK in human oral squamous cell carcinomas (OSCC) and normal oral epithelium (NOE) using immunohistochemistry, laser confocal microscopy and Western blotting. The subcellular localization of BRK was identified by confocal microscopy and Western blotting of nuclear and cytoplasmic extracts from these cells. The results indicate that NOE express higher Levels of BRK compared with OSCC cells. In NOE and moderately differentiated OSCC cells, BRK was localized in the nucleus and cytoplasm. However, in poorly differentiated OSCC cells, BRK was localized in perinuclear regions. These results suggest that BRK expression differs in normal and OSCC which may reflect a possible functional involvement in OSCC. (C) 2004 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available