Journal
BIOCONJUGATE CHEMISTRY
Volume 15, Issue 6, Pages 1297-1303Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc049855h
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Cross-linking reagents based on an azobenzene core can be used to reversibly photoregulate secondary structure when introduced as intramolecular bridges in peptides and proteins. Photoisomerization of the azobenzene core in the trans to cis direction is triggered by photon absorption but isomerization from cis to trans occurs thermally as well as photochemically. The rate of the thermal process effectively determines the half-life of the cis form as well as the extent to which the trans form can be recovered. We designed and characterized a series of methanethiosulfonate (MTS)-bearing thiol-reactive azobenzene-based cross-linkers. These cross-linkers are shown to permit photoregulation of helix content in a test peptide with half-lives for the cis conformation ranging from 11 s to 43 h at 25 degreesC. The cross-linkers described here thus broaden the range of reagents available for reversible photocontrol of peptide and protein conformation.
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